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人Egr-1启动子的克隆及其在肿瘤细胞中的辐射诱导反应 被引量：2

Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells
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摘要 目的:克隆人Egr-1基因的启动子,插入荧光素酶报告基因载体中,并检测电离辐射对其活性的影响。方法:采用PCR技术从人乳腺癌细胞系MCF-7基因组中扩增出Egr-1启动子,将其克隆到pGL3-basic载体中;将重组质粒转染人肿瘤细胞,测定Egr-1启动子在不同辐射条件下转录活性的改变。结果:成功构建了Egr-1启动子的荧光素酶报告基因;在不同剂量的γ射线照射后,Egr-1的启动子活性均明显高于未照射组;在同一剂量照射后48 h,Egr-1的启动子活性达峰值。结论:本实验构建的Egr-1启动子具有辐射激活的功能,为进一步研究放射-基因治疗奠定了基础。 AIM： To construct human Egr-1 promoter luciferase reporter system and study its activity induced by ionizing radiation. METHODS： Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay. RESULTS： The luciferasy reporter system of Egr-1 promoter was successfully constructed. The activity of Egr-1 promoter was substantially increased after different doses of IR and reached to the peak at the time point of 48h after IR. CONCLUSION： The Egr-1 promoter was constructed in this study showed IR inducible activity in tumor cells, laying foundation for the research of radiation, mediated gene therapy.
作者 徐小洁 丁丽华 王凌雪 秦玺 程龙 蒋凯 叶棋浓
机构地区 军事医学科学院生物工程研究所
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2009年第11期973-975,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 全军医药卫生科研基金(06J021) 国家重大科学研究计划(2007CB914603)
关键词 EGR-1启动子 辐射 启动子活性 Egr-1 promoter ionizing radiation promoter activity
分类号 R392.11 [医药卫生—免疫学]
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参考文献7

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共引文献2

同被引文献19

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细胞与分子免疫学杂志
2009年 第11期

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