MMP-2和VEGF-C在甲状腺乳头状癌中的表达及临床意义

Expressions and Clinical Significance of MMP-2 and VEGF-C in Papillary Thyroid Carcinoma

目的探讨甲状腺乳头状癌中基质金属蛋白酶-2(MMP-2)和血管内皮细胞生长因子-C(VEGF-C)的表达及其临床意义。方法应用免疫组织化学SP法检测78例甲状腺乳头状癌和同期18例甲状腺良性肿瘤组织中MMP-2和VEGF-C的表达情况,并进行比较分析。结果MMP-2和VEGF-C在甲状腺乳头状癌组织中的表达阳性率(80.77%和75.64%)明显高于甲状腺良性肿瘤(11.11%和22.22%),P<0.05。MMP-2和VEGF-C的表达与甲状腺乳头状癌的浸润程度和颈淋巴结转移有关,即肿瘤浸润达包膜至包膜外者或临床有颈淋巴结转移者,其表达阳性率明显高于肿瘤浸润局限在包膜内者或临床无颈淋巴结转移者(P<0.05);同时临床无颈淋巴结转移的41例患者在术后随访中,出现颈淋巴结转移者其表达阳性率亦明显高于未出现转移者(P<0.05)。MMP-2和VEGF-C在甲状腺乳头状癌组织中的表达呈正相关(Gamma=0.846,P<0.05)。结论MMP-2和VEGF-C对甲状腺良、恶性肿瘤的鉴别有一定帮助;MMP-2和VEGF-C可以作为预测甲状腺乳头状癌浸润转移的分子生物学指标之一;两者具有协同作用,联合检测对预测颈淋巴结转移状况有一定的指导意义。

Objective To study the expressions and clinical significance of matrix metalloproteinases-2 （MMP 2） and vascular endothelial growth facto-C （VEGF C） in patients with papillary thyroid carcinoma （PTC）. Methods SP immunohistochemical technique was used to detect the expressions of MMP-2 and VEGF-C in 78 cases of PTC and 18 cases of thyroid benign tumors. Results The positive expression rates of MMP-2 and VEGF-C in PTC （80.77 %, 75.64 % ） were significantly higher than those of the thyroid benign tumor （ 11.11%, 22.22 % ）, P〈0. 05. The expressions of MMP-2 and VEGF-C were correlated to the degree of infiltration and lymph node metastasis in PTC： In those which infiltrated to or over the thyroid capsular, or had clinical neck lymph node metasta sis, the positive expression rates were significantly higher than those in the other cases which had confined invasion of thyroid capsular or non clinical metastasis of neck lymph node （P〈0.05）. And during the follow up of 41 patients who didn＇t have clinical neck lymph node metastasis before operation, the positive expression rates of those who had clinical neck lymph node metastasis were significantly higher than those in the other patients who didn＇t have neck lymph node metastasis （P〈0.05）. There was significantly positive correlation between the expressions of MMP 2 and VEGF-C in PTC （Gamma=0. 846, P〈0.05）. Conclusions MMP-2 and VEGF-C may be used to distinguish malignant and benign thyroid tumor; The expressions of MMP-2 and VEGF-C are correlated with the degree of infiltration and neck lymph node metastasis in PTC; Combined detection of MMP2 and VEGF-C will be more accurate to predict condition of lymph node metastasis.