基因芯片检测人乳头瘤病毒在子宫内膜癌组织中的感染状况

Clinical Study of Gene Chip for Human Papillomavirus Genotyping in Endometrial Carcinoma

目的:了解人乳头瘤病毒(HPV)在子宫内膜癌患者子宫内膜组织中的感染状况。方法:采用套式PCR扩增HPV DNA和HPV基因分型芯片技术,对40例子宫内膜癌患者、20例异常子宫出血患者和20例正常子宫内膜组织进行HPV基因亚型分析。结果:40例子宫内膜癌患者的子宫内膜石蜡包埋组织2例HPV DNA阳性,检出率为5.0%(2/40),均为HPV6型感染;20例异常子宫出血患者的子宫内膜组织3例HPV DNA呈阳性,检出率为15.0%(3/20),分别为HPV6型、HPV11型及HPV11型和HPV6型混合感染;20例正常子宫内膜组织无HPV DNA阳性表达,检出率为0(0/20)。结论:子宫内膜组织中HPV感染较少,可能不是HPV复制、成熟的适宜宿主上皮,子宫内膜的癌前病变和子宫内膜癌的发生发展可能与HPV感染无关。

Objective：To study the relationship between endometrial carcinoma and human papillomavirus （HPV） infection. Methods： The paraffin-embedded tissues collected from 40 patients with endometrial carcinoma, 20 patients with abnormal uterine bleeding and 20 normal controls were detected using nested-PCR and Gene Chip. Results：HPV was detected in 2 of specimens among the 40 cases of endometrial carcinoma. The positive rates of HPV infection in endometrial carcinoma were 5.0%（2/40）. HPV was detected in 3 of specimens of 20 patients with abnormal uterine bleeding. The positive rates of HPV infection were 15.0%（3/20）in patients with abnormal uterine bleeding. There was no HPV DNA in the controls （0/20）. Conclusion： HPV being hardly detected in endometrium indicates that endometrium is not the suitable host of HPV replication and maturation. HPV infection seems not related to pathogenesis and transformation process of endometrial carcinoma.