摘要
目的:观察抑郁症模型大鼠血清雌二醇(E2)水平、海马5-羟色胺(5-HT)含量及其中缝核雌激素受体β(ERβ)和色氨酸羟化酶2(TPH2)表达的变化,探讨抑郁症发生的可能机制。方法:雌性SD大鼠20只,随机平均分为正常组和模型组,模型组给予孤养和21d慢性不可预见性的温和应激(CUMS)制备抑郁症大鼠模型。采用放射免疫分析法(RIA)测定血清雌二醇(E2)水平、高效液相色谱-电化学检测法(HPLC-ECD)测定海马5-羟色胺(5-HT)含量、RT-PCR技术检测中缝核ERβmRNA和TPH2mRNA表达以及荧光免疫组化双重染色法检测中缝核ERβ蛋白和TPH2蛋白表达。结果:21d应激后,模型组大鼠血清E2水平下降、海马5-HT含量减少、中缝核ERβmRNA和TPH2mRNA相对表达量下降及ERβ阳性细胞数和TPH2阳性细胞数明显减少。结论:E2与海马5-HT之间可能存在E2→中缝核5-HT能神经元ERβ→TPH2→海马5-HT的功能作用链。
Objective： To investigate the changes of serum estradiol （E2）, the concentration of 5-hydroxytryptamine （5- HT） in hippocampus and the expression of estrogen receptor 13 （ER13） and tryptophan hydroxylase 2 （TPH2） in median raphe nuclei in depression model of rats ,and the mechanism thereof. Methods： The animal model was established by giving isolation-housing in combination with 21 days of chronic unexpected mild stress （CUMS）. E2 was measured by radio-immunoassay （RIA）. The content of 5-HT in the hippocampus was determined by high performance liquid chromatogram-eleetrochemical detection （HPLC-ECD）. The relative expression of ERI3 mRNA or TPH2 mRNA was estimated with hemi-quantitative reverse transcription - polymerase chain reaction （RT-PCR） technique, and the number of ER13 or TPH2 immunoreactive neurons and their collocation state were detected with dual-label immunofluorescence method. Results： After 21 days of CUMS, the level of serum E2 was lowered in model group. The concentration of 5-HT in hippocampus was decreased, and the relative expression of ERI3 mRNA, TPH2 mRNA and numbers of ER13 and TPH2 imnmnoreactive neurons were decreased in raphe nuclei as well. Conclusion： The effect of E, on 5-HT might be mediated through ER13 and TPH2 in the raphe nuclei, and then led to affect 5- HT content in hippocampus.
出处
《天津医药》
CAS
北大核心
2009年第11期944-947,I0001,共5页
Tianjin Medical Journal
基金
江西省自然科学基金资助项目(项目编号:2007GQY0944)
