胃癌中幽门螺杆菌与凋亡相关蛋白p53、BcL-2、Fas表达相关性研究

Relationship between Hp and apoptosis-related proteins p53,BcL-2 and Fas expressions in gastric cancer

目的:探讨幽门螺杆菌(Hp)感染在胃癌发生、发展中与p53基因、BcL-2基因、Fas基因表达的关系。方法:用Warthin-Starry染色法和快速尿素酶试验观察在不同胃病黏膜组织中Hp的感染情况,用免疫组化S-P法检测p53、BcL-2、Fas基因蛋白的表达。结果:胃癌及癌前病变(IM、Dys)组中Hp感染率明显高于慢性胃炎(CG)组,差异有统计学意义(P<0.05)。p53、Bcl-2的阳性表达率在GC、Dys和IM组中明显高于CG组,差异有统计学意义(P<0.05);Fas在GC及Dys组中表达明显低于CG组,差异有统计学意义(P<0.05)。在GC、Dys及IM中,Hp感染(+)组中p53、Bcl-2基因蛋白表达阳性率高于Hp感染(-)组,Fas基因蛋白的表达低于Hp感染(-)组,差异有统计学(P<0.05)。结论:Hp与胃癌的发生关系密切,在胃癌的发生发展过程中,Hp感染与细胞凋亡调控蛋白p53、Bc1-2及Fas之间存在相互协同的关系。

Objective To investigate the relationship between Hp infection and p53,BcL-2,Fas expressions in gastric cancer.Method Infection of Hp in different gastric mucosa were observed by W-S staining and rapid urease test.p53,BcL-2 and Fas protein expressions were determined by immunohistochemical technology.Results The expressions of Hp were higher in gastric carcinoma group and precancerous lesion group than chronic gastritis group（P〈0.05）.The expressions of p53 and Bcl-2 were higher in GC,Dys and IM group,compared with CG group（ P 〈 0.05 ）. The expressions of Fas were lower in GC and Dys group than CG group （ P 〈 0.05 ）. The expressions of p53 and Be1-2 in Hp（ ＋ ） group were higher than those in Hp（ - ） group （P 〈0.05） ,Fas in Hp （ - ） group were higher than those in Hp（ ＋ ） group （P 〈0. 05）. Conclusion The development of gastric carcinoma has close relations with Hp infection. Hp infeetion and apoptosis - related proteins p53, BcL - 2 and Fas have synergetic relations in the development of gastric carcinoma.