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API-2靶向抑制PI3K/Akt信号通路干预胃癌细胞生长的研究 被引量：1

Effects of Selective Inhibition by API-2 on the Growth of Gastric Cancer Cells
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摘要 目的研究蛋白激酶B抑制剂-2(API-2)靶向抑制PI3K/Akt信号通路中Akt位点,从而对胃癌细胞生长的影响。方法使用API-2处理人胃癌细胞株SGC7901。MTT法检测0,2,4,6,8μmol/LAPI-2作用不同时间对细胞增殖的影响;荧光染料AO/EB染色观察不同浓度API-2作用24h后细胞凋亡情况;流式细胞仪检测细胞周期变化;Western blot法检测p-AKT蛋白以及下游蛋白NF-κB表达情况。结果API-2可抑制SGC7901细胞的增殖,并呈浓度和时间依赖性;不同浓度API-2作用24h后,对细胞的部分周期有明显的影响,也可直接导致肿瘤细胞凋亡、坏死,并且p-AKT蛋白及下游蛋白NF-κB表达均减少。结论API-2有效地抑制PI3K/Akt信号通路中Akt位点,影响SGC7901细胞的周期,减少NF-κB的表达,具有增殖抑制及诱导凋亡作用。 Objective To study the effect of the inhibition of Akt by API-2 in PI3K/Akt signaling pathway on the growth of gastric cancer cells. Methods SGC7901 cells were treated with API-2. The effect of API-2 （0,2,4,6,8 μmol/L） on their proliferation was observed with MTT assay. Cell apoptosis after treated with API-2 for 24 h was observed by AO/EB double staining for fluorescence microscopy. Cell cycle was analyzed by flow cytometry （FCM）. The expression of p-AKT and NF-κB was detected by Western blot. Results API-2 （0,2,4,6,8 μmol/L） significantly inhibited the proliferation of SGC7901 cells in dose-and time-dependent manner. When treated with different densities of API-2 for 24 h, parts of the cell cycle of SGC7901 cells were obviously blocked. Apoptosis and necrosis of the tumor ceils were increased while p-AKT and NF-κB expression was decreased. Conclusion API-2 can inhibit proliferation and induce apoptosis of SGC7901 cells by effectively inhibiting Akt in PI3K/Akt signaling pathway, affecting cell cycle distributions of the cells, and greatly reducing the expression of NF-κB.
作者 黄昌明 吴日平
机构地区 福建医科大学附属协和医院肿瘤科
出处 《福建医科大学学报》 2009年第5期373-376,共4页 Journal of Fujian Medical University
基金 福建省教育厅B类科技项目(JS07009)
关键词 胃肿瘤 蛋白酶抑制药 1-磷脂酰肌醇3-激酶 蛋白质丝氨酸苏氨酸激酶 NF-κB 信号传导 细胞凋亡 肿瘤细胞 培养的 stomach neoplasms protease inhibitors 1-phosphatidylinositol 3-kinase protein- serine-threonine kinases NF-kappa B signal transduction apoptosis tumor cells, cultured
分类号 R735.202 [医药卫生—肿瘤] R329.24 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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同被引文献13

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  • 10杨宁,王恬,惠起源.PI3K-Akt信号通路与胃癌的研究进展[J].现代肿瘤医学,2010,18(1):197-200. 被引量：16

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福建医科大学学报
2009年 第5期

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