高压氧对鼻咽癌细胞生长与氧自由基的影响

Effects of hyperbaric oxygen on the growth and oxygen free radical of nasopharyngeal carcinoma cells

目的通过观察高压氧处理后人鼻咽癌细胞系CNE-2细胞生长抑制率和死亡率及超氧化物歧化酶（SOD）、丙二醛（MDA）含量的变化，初步探讨高压氧处理对鼻咽癌细胞生长的影响及其机制。方法将实验培养的人鼻咽癌CNE-2细胞分为A组（对照组）、B组（高压氧0．15MPa）、C组（高压氧0．20MPa）、D组（高压氧0．25MPa）和E组（高压氧0．30MPa）。采用MTT法分析细胞生长抑制率，PI染色观察细胞死亡情况，并采用黄嘌呤氧化酶法测定SOD的含量、硫代巴比妥法测定MDA的含量。结果高压氧处理各组的细胞死亡率、MDA含量均较A组明显增加，比较差异均有统计学意义（P〈0．05）；各组鼻咽癌CNE-2细胞生长抑制率、死亡率和MDA含量比较差异有统计学意义（P〈0．05），且均随高压氧处理压力升高而增加。结论高压氧处理增加实验培养的人鼻咽癌CNE-2细胞生长抑制率、死亡率，可能与高压氧处理增加鼻咽癌CNE-2细胞内氧自由基有关。

Objective To investigate the effect and possible influential mechanism of hyperbaric oxygen（HBO） on the growth and death of human nasopharyngeal carcinoma（NPC） cell line--CNE- 2 by observing the inhibition rate of growth and mortality rate and the content of superoxide dismutasc （ SOD ） and malondialdehyde （MDA） of nasopharyngeal carcinoma cells through HBO treatment. Methods Nasopharyngeal carcinoma （ NPC ） cell line--CNE - 2 were randomly divided into 5 groups ： Group A （ the control） ; Group B （ hyperbaric oxygen treatment at 0.15 MPa） ; Group C （ hyperbaric oxygen treatment at 0.20 MPa） ; Group D： （hyperbaric oxygen treatment at 0.25 MPa） ; Group E （hyperbaric oxygen treatment at 0.30 MPa）. Inhibition rates of growth in CNE 2 cells of all groups were detected by MTT reduction assay, mortality rates were detected by PI staining, and the content of SOD and MDA were also detected with xanthine oxidase detection assay and thiobarbital detection assay. Results Mortality rate and content of MDA of all the HBO treatment groups increased dramatically when compared with those of Group A （ P 〈 0. 05 ）. Statistical differences could be noted in contents of MDA, inhibition rates of growth and mortality rates in all the groups （P 〈 0.05）. And the rate of increase was in proportional to the increase of HBO pressure. Conclusions HBO treatment could increase inhibition rates of growth and mortality rate of human NPC cell line--CNE - 2, which might be correlated with the increase in oxygen free radicals in human nasopharyngeal carcinoma cells through HBO treatment.