胰岛素促进慢性创面愈合的效果及机制

Mechanism and effect of insulin on chronic wound

目的:研究胰岛素治疗慢性创面的效果和机制,为胰岛素临床治疗慢性创面提供理论依据。方法:45只SD大鼠制作慢性创面模型,随机分为对照组(A组)、胰岛素4单位组(B组)和8单位组(C组),每组15只。损伤后第8天,测量各组创面面积、血糖,并应用RT-PCR方法检测各组VEGF表达情况。结果:创面面积及血糖A组与B组和C组相比,有明显统计学差别(P<0.05);而B组和C组之间无统计学差别(P>0.05);同A组相比,B组和C组VEGFmRNA表达有明显增高的趋势。结论:胰岛素有可能通过上调VEGF表达,促进创面愈合。

Objective：To investigate the effect and mechanism of insulin used on wound surface to promote chronic wound healing and clinical application of insulin on treatment of chronic wound. Methods： Animal model of chronic wound was made in 45 SD rats and all the animals were divided into three groups ：A（control group） ,B（4 U group） and C（8 U group） randomly. In the eighth day,the area of the wound surface and the blood sugar values were measured. In addition, the express of VEGF was measured by using RT - PCR. Results： The curative effects in groups B and C were better than effects in group A. The area of the wound surface and the blood sugar values in groups B and C were significantly different as compared with group A （P 〈 0. 05）. Moreover, the express of VEGF in groups B and C were significantly higher than those in group A （ P 〈 0. 05 ）. Conclusion： Applying low dose of insulin on local wound surface could promote healing of chronic wound by increasing the expression of VEGF.