流式细胞术检测胃癌患者腹腔冲洗液游离癌细胞的变化

Changes of free cancer cells detected by flow cytometry in peritoneal lavage of gastric cancer patients after gastrectomy

目的:探讨胃癌手术对腹腔脱落癌细胞的影响。方法:收集50例行胃癌手术患者胃癌切除前后腹腔冲洗液,以流式细胞学(FCM)方法检测腹腔冲洗液癌胚抗原(CEA)及角蛋白-19(CK-19),同时采用HE染色进行腹腔冲洗液细胞学(PLC)检查。结果:胃癌手术患者CEA检查手术前后阳性率分别为46%(23/50)和64%(32/50),CK-19检查手术前后阳性率为48%(24/50)和68%(34/50),两组之间差异有统计学意义(P<0.05)。PLC检查结果手术前后分别为20%(10/50)和36%(18/50)(P<0.05)。术前、术后PLC阳性者FCM均为阳性。结论:胃癌手术增加腹腔胃癌细胞的脱落。

Objective：To investigate the effect of gastrectomy on gastric cancer cells in peritoneal lavage fluid from patienm with gastric cancer. Methods ： Peritoneal lavage fluid from 50 patients with gastric cancer was collected before and after gatrectomy to detect carcinoembryonic antigen （CEA） and cytokeratin - 19 （ CK - 19 ） using FCM. The peritoneal lavage cytology examination（PLC） was carried out by HE staining. Results：The pre - ,and postoperative CEA positive rates were 46% （ 23/50 ） and 64% （ 32/50 ）, respectively, and there was significant difference （ P 〈 0.05 ） ; while, the pre -, and postoperative CK - 19 positive rates were 48% （24/50）and 68% （34/50）with significant difference （ P 〈 0.05 ）. The pre - , and postoperative PLC positive rates were 20% （10/50） and 36 % （18/50） （ P 〈 0.05 ）. Conclusion：Gastrectomy increases the intraperitoneal free cancer cells.