高强度聚焦超声治疗对结直肠癌肝转移患者T淋巴细胞亚群和NK细胞的影响

Effect of high intensity focused ultrasound on T-lymphocyte subsets and natural killer cells in patients of hepatic metastases from colorectal cancer

目的:探讨高强度聚焦超声治疗(HIFU)对结直肠癌肝转移患者免疫功能的影响。方法:32例结直肠癌肝转移患者52个病灶经HIFU治疗,治疗前和治疗后4周分别抽取外周血,流式细胞仪检测NK细胞(CD16+、CD56+)、CD4+、CD8+T细胞百分数和CD4+/CD8+值。结果:经HIFU治疗后,结直肠癌肝转移患者的NK细胞百分数升高(P<0.05),CD4+T细胞百分数和CD4+/CD8+比值升高(P<0.01),CD8+T细胞百分数则下降(P<0.01)。随访观察32例病人52个病灶中,35个病灶肿瘤体积缩小50%,17个病灶肿瘤体积保持稳定。结论:高强度聚焦超声治疗可以改善结直肠癌肝转移患者的细胞免疫功能。

Objective：To evaluate the effect of high intensity focused ultrasound （HIFU） therapy on T- lymphocyte subsets and natural killer（NK） ceils in patients of hepatic metastases from colorectal cancer. Methods： All 52 leisions in 32 cases of hepatic metastases from colorectal cancer were treated by HIFU therapy. The percentages of NK cells（CD16 ＋ ,CD56 ＋ ）, CIM ＋ and CD8 ＋ T cells, the ratio of CIM ＋ to CD8 ＋ in peripheral blood were checked by flowc）rtometry before and 4 months after treatment respectively. Results： With the treatment of HIFU, the percentage of NK cells increased（ P 〈0.01 ） ,the percentage of CD4 ＋ T cells and the ratio of CD4＋ to CD8 ＋ also elevated（ P 〈 0.01 ） ,while the percentage of CD8 ＋T cells decreased（P 〈 0.01 ）. Conelusion：HIFU therapy can improve the cell immunity function of the patients of hepatic metastases from colorectal cancer.