急性血栓性肺栓塞兔血清肝细胞生长因子的变化及其意义

Changes and significance of hepatocyte growth factor in plasma of rabbit after pulmonary thromboembolism

目的:观察急性血栓性肺栓塞(acute pulmonary thromboembolism,PTE)兔血清中肝细胞生长因子(hepatocyte growth factor,HGF)的变化特征,并探讨其对PTE的早期诊断价值。方法:新西兰纯种白兔16只,随机均分为对照组和模型组;模型组采用自体血栓回输法建立PTE模型,对照组以等量0.9%氯化钠溶液代替。分别在0、1、3、6、12、24、48、72h采集血样,ELISA法检测HGF及IL-1β含量。结果:模型组血HGF水平于1h后开始升高[(0.853±0.317)μg/L],持续升高到3h略降低后又继续升高,12h升高最明显[(1.742±0.487)μg/L],在48h仍保持高水平,72h开始回落;对照组各时间点血HGF水平均无明显变化,一直保持在较低水平;模型组在1、3、6、12、24、48、72h血清HGF水平均显著高于对照组(P<0.05～0.01)。模型组血IL-1β于0h后开始升高,在3h达到峰值(0.181μg/L),6h降至较低水平(0.09μg/L),直至72h无明显变化;对照组始终在<0.089μg/L水平。结论:HGF有望作为一种新的生物学标记物用于PTE早期诊断。

Objective：To study the feature of hepatocyte growth factor （HGF） in the plasma of rabbit with acute pulmonary thromboembolism（PTE）, and to explore the early diagnostic value to PTE. Method： Total of 16 rabbits were randomly divided into blank and PTE groups with 8 in each group,the PTE model was estabished by intravenous injection autologous blood clots, the sham-opreated groups was instead with NS. The level of HGF and IL-1β （0,1,3,6,12,24,48,72 h） was detected by ELISA. Result.. The plasma HGF level between PTE group and blank group was signaficant（P〈0. 05）, and began to elevated at 1 h after PTE, HGF was markedly increased at 6 h,12 h,24 h and 48 h in PTE groups,signaficantly elevated at 12 h（[1. 7425=0. 487]μg/L,P〈0. 01）, then deceased at 72 h. The blank groups was below 0. 5 μg/L. IL-1βreached the maximum level at 3 h （0. 181 μg/L）. Conclusion：The HGF may be a new biol-marker for the early diagnosis of PTE.