大肠癌中pAkt、VEGF和MVD的检测及其临床意义

Significance of and pAkt,VEGF and intratumour MVD in colorectal carcinomas

目的检测大肠癌组织磷酸化丝氨酸/苏氨酸激酶(pAkt)、血管内皮生长因子(VEGF)的表达和微血管密度(MVD)及其临床意义。方法应用免疫组化SP法检测76例大肠癌的pAkt、VEGF和MVD。结果大肠癌pAkt和VEGF的阳性表达率分别为73.7%(56/76)和85.5%(65/76)。pAkt的阳性表达与肿瘤浸润深度、临床分期和淋巴结转移(P<0.05)及其MVD(P<0.01)显著相关。pAkt阳性组的MVD明显高于阴性组。VEGF的阳性表达与大肠癌的临床分期显著相关(P<0.05)。pAkt蛋白表达与VEGF蛋白表达密切相关(P<0.05)。结论pAkt和VEGF与大肠癌发生、发展和转移密切相关,提示阻断pI3K/Akt信号传导通路将对大肠癌的治疗提供新的靶点。

Objective To study effect of the expression of phosphorylated Akt （pAkt）, vascular endothelial growth factor （VEGF） and on intratumour microvessel density （MVD）in colorectal carcinoma tissues. Methods method was used to detect the expression of pAkt, VEGF and MVD in 76 colorectal carcinomas. Results The positive expression rates of pAkt and VEGF of colorectal carcinoma were 73.7 % （56/76） and 85.5 % （65/76） respectively. The positive expression of pAkt was correlated to the invasive depth, clinical staging, lymph node metastasis and intratumor MVD. The positive expression of VEGF was closely related to clinical staging. The expression of pAkt and VEGF was positively correlation. Conclusion The results shown that pAkt and VEGF might have important significance in the carcinogenesis, progression, and metastasis of colorectal carcinoma, and pI3K/Akt might be as a potentially useful target for therapeutic intervention in colorectal carcinoma patients.