淡豆豉异黄酮对增殖血管平滑肌细胞JAK2/STAT3信号转导通路的影响

Effect of Semen Sojae Preparatum isoflavone on JAK2/STAT3 signal transduction pathway in proliferated vascular smooth muscle cell

目的探讨淡豆豉异黄酮对血管紧张素Ⅱ(angiotensin Ⅱ,AngⅡ)诱导大鼠血管平滑肌细胞(vascular smooth muscle cell,VSMC)增殖及Janus激酶2-细胞信号转导及转录活化因子3(JAK2/STAT3)信号通路的影响。方法建立AngⅡ诱导VSMC增殖模型,MTT法检测VSMC增殖活性;RT-PCR法检测AngⅡ1型受体(angiotensin Ⅱreceptor1,AT1R)mRNA表达;Westernblot法检测JAK2/STAT3信号通路蛋白JAK2,STAT3及其磷酸化表达水平。结果100、200μg·L-1淡豆豉异黄酮可明显抑制AngⅡ诱导的VSMC增殖,并明显下调AT1R mRNA表达水平;200μg·L-1淡豆豉异黄酮可明显下调磷酸化JAK2、磷酸化STAT3的表达。结论淡豆豉异黄酮可抑制AngⅡ诱导的VSMC增殖,其机制可能与下调AT1R表达,阻断JAK2/STAT3信号通路的磷酸化有关。

Aim To study the effect of Semen Sojae Preparatum isoflavone（SSPI）on proliferation and JAK2/STAT3 signal transduction pathway in rat vascular smooth muscle cell（VSMC）induced by Angiotensin Ⅱ（AngⅡ）.Methods A cell proliferating model of VSMC induced by AngⅡ was established.The proliferation activity of VSMC was analyzed by MTT method.The expressions of angiotensin Ⅱ receptor 1（AT1R） were detected by RT-PCR method.The expressions of JAK2,STAT3 and phosphorylation protein were detected by Western blot.Results 100 μg·L-1, 200 μg·L-1 SSPI significantly inhibited the proliferation of VSMC induced by AngⅡ,and down-regulated the mRNA expression of AT1R.200 μg·L-1 SSPI could significantly down-regulate the protein expressions of p-JAK2,p-STAT3.Conclusions The proliferation of VSMC induced by AngⅡcan be inhibited by SSPI.The mechanisms might be related to down-regulating the expressions of AT1R,and arresting the phosphorylation of JAK2/STAT3 signal transduction pathway.