摘要
目的观察辛伐他汀(simvastatin)对白细胞介素-18(interleukine-18,IL-18)诱导人脐静脉内皮细胞(HUVECs)上调表达Fractalkine(FKN)的影响以及对FKN黏附作用的干预效应。方法应用逆转录-聚合酶链反应(RT-PCR)检测HEUVCs FKN的表达;应用Flowchamber观察FKN介导的HUVECs与单核细胞THP-1的黏附。结果IL-18可诱导FKN mRNA表达增加,FKN表达上调可明显增加对单核细胞THP-1的黏附作用(P<0.01);PDTC能够抑制FKN表达及细胞黏附(P<0.01);辛伐他汀能够抑制FKN mRNA表达(P<0.01),100μmol·L-1辛伐他汀可抑制细胞黏附(P<0.01)。结论辛伐他汀能够抑制FKN的表达以及FKN介导的黏附作用。
Aim To investigate the effect of simvastatin on the FKN expression in human umbilical vascular endothelial cells（HUVEC）up-regulated by interleukine-18（IL-18）,and the effect on adhesion of FKN to monocyte THP-1.Methods FKN expression in HUVEC was determined by reverse transcription-polymerase chain reaction （RT-PCR）, and the adhesion was checked by in vitro Flow chamber.Results Incubation of HUVECs with IL-18 upregulated FKN mRNA expression（P0.01）,The up-regulated expression of FKN enhanced the adhesion of THP-1 cells to HUVECs（P0.01）.PDTC inhibited expression of FKN mRNA and the adhesion（P0.01）;simvastatin inhibited FKN mRNA expression,100 μmol· L-1 simvastatin inhibited adhesion（P0.01）.Conclusion Simvastatin plays an important role in influencing the expression and adhesion of FKN.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第11期1497-1502,共6页
Chinese Pharmacological Bulletin
