河北石家庄2005-2007年甲肝病毒流行株基因分型研究

Genetic analysis of HAV strains isolated from patients with acute hepatitis in Hebei Shijiazhuang of China, 2005-2007

目的了解石家庄地区甲型肝炎病毒（HAV）流行株基因型特征，为HAV溯源研究打下基础。方法收集了2005--2007年石家庄地区部分甲肝患者急性期血清标本，用HAV结构．非结构区VP1—2A基因引物，经核酸提取，RT-PCR，序列测定，对HAV进行基因分型分析。结果石家庄地区2005--2007年HAV流行株VP1-2A区核苷酸序列同源性为95％～100％，都属于IA亚型；该区氨基酸序列几乎相同。结论石家庄地区存在有多株甲肝病毒流行株，同一株甲肝病毒可以存在不同地区，同一地区可以检测到相同或不相同的HAV毒株。为今后进一步开展甲肝病毒分子流行病学研究及有效控制HAV流行提供了理论和技术支撑。

Objective To characterize the hepatitis A virus （HAV） wild type strains circulating in Hebei Shijiazhuang of China during 2005-2007, to provide the bases for further investigation of the sources of HAV infection. Methods The VP1/P2A junction regions were detected by RT-PCR from HAV IgM positives serum samples during 2005 and 2007, the 34 RT-PCR positive samples were sequenced and subjected to phylogenetic analysis by Neighbor Joining （N J） method. Results All the detected HAV strains were identified as sub-genotype I A, the homology of nucleotide sequence in the VP1-2A imction region ranged from 95%-100%, the amino acid sequences of HAV strains almost had no difference. Conclusion There are different HAV strains existing in Hebei Shijiazhuang of China, same HAV strain may exist in different areas; or in one area, identical or different HAV strains may be detected. This work provides the bases for further investigation of the sources of HAV infection and also for effectively control measures to prevent the spread of the disease.