新疆和田2006年甲肝病毒流行株基因分型研究

Genotyping of Hepatitis A virus prevalent strains in Xinjiang Hetian of China in 2006

目的了解2006年新疆和田甲型肝炎病毒（HAV）流行株基因型特征，为HAV溯源研究打下基础。方法收集了新疆和田部分甲肝病人血清标本，用HAV结构．非结构区基因VPI-2A引物，经核酸提取，RT-PCR，序列测定，对HAV进行基因分型研究。结果新疆和田VP1—2A区HAV核苷酸序列变异为0％-3．9％，分为不同基因簇，但都属1A亚型；VP1-2A区氨基酸序列只有0—2个差异。与已发表的2005年新疆伊犁部分甲肝病毒流行株基因有相同序列或同源性较高。结论和田有多株甲肝病毒存在，本研究流行可能有多个传染源，多个传播链，在人群免疫水平较低时，引起甲肝流行。结果表明HAV分子流行病学方法在HAV流行株遗传变异及溯源研究中，以及控制HAV流行中具有重要作用。

Objective To analysis the genotypes of wild type hepatitis A virus circulated in Xinjiang Hetian of China in 2006. Methods The Vpl-2A region of HAV genome was amplified and sequenced from serum samples collected in Xinjiang Hetian of China in 2006, and subjected to phylogenetic analysis by Neighbor Joining （NJ） method. Results The nucleotide sequence differences in the VP1-2A region among Xinjiang Hetian HAV strains ranged from 0%-3.9%, all belonged to sub-genotype 1A. Genetically similar strains were identified among Xinjiang Hetian 2006 and Xinjiang Yili 2005 of China isolates. Only 0-2 amino acid differences were found among the Xinjiang Hetian HAV isolates in the VP1-2A region. Conclusion There were different HAV strains existing in the investigated areas, these strains may have different transmission pathways for the spread of the disease. The results indicate the usefulness of molecular epidemiological methods in studying changes in the circulating HAV strains and in tracing transmission routes, and also for effectively control measures to prevent the spread of the disease.