慢性重型乙型肝炎与HLA—DRB1基因多态性的相关性研究

Study on the association between severe chronic hepatitis B and HLA-DRB1 gene polymorphism

目的探讨慢性重型乙型肝炎患者与HLA—DRB1基因多态性的相关性。方法采用聚合酶链反应／序列特异性引物技术对河南地区26例慢性重型乙型肝炎患者和45例健康对照者进行HLA—DRB1等位基因的检测，应用统计学软件SPSS13．0对两组实验结果进行χ^2检验。结果在慢性重型乙型肝炎组中，HLA—DRB1＊1301／1302出现的频率明显高于对照组，差异有统计学意义；HLA—DRB1＊1501／1502，＊1201／1202在两组间出现的频率相比差异无统计学意义。结论HLA-DRB1＊1301／1302等位基因可能和慢性重型肝炎的发生有关，HLA—DRB1＊1501／1502，＊1201／1202与慢性重型肝炎的发生无相关性。

Objective To study the association between HLA-DRB1 gene polymorphism and severe chronic hepatitis B. Methods 26 patients with severe chronic hepatitis B were investigated for HLA-DRB1 gene polymorphism by polymerase chain reaction-sequence specific primers technique. The results were compared with those from 45 normal healthy people by use of χ^2-test of Microsoft SPSS 13.0. Results The frequency of HLA- DRB1 ＊ 1301/1302 allele in severe chronic hepatitis B group was significantly higher than the frequency in the control group, while the frequencies of HLA-DRB1 ＊ 1201/1202, 1501/1502 allele were not significantly different. Conclusion HLA-DRB1 ＊ 1301,1302 is closely associated with the suseptibility to severe chronic hepatitis B, While HLA-DRB1 ＊ 1201/1202, 1501/1502 have no association with severe chronic hepatitis B.