一种检测腹泻相关病毒的方法

A method of detect the virus which caused diarrhea

目的建立检测腹泻粪便标本中病毒病原的方法。方法选取本实验室检测的杯状病毒、肠道腺病毒、轮状病毒、博卡病毒、星状病毒以及肠道病毒阳性标本各一份，通过非序列依赖性单一PCR扩增（Sequence—independent single primer amplification，SISPA）构建基因文库，从中筛查病毒基因片段。结果六份标本中都可以得到相对应的病毒核酸序列。结论本研究采用的方法可以检测到粪便标本中引起腹泻的相关病毒，为进一步研究检测目前尚未发现的病毒病原奠定基础。

Objective Building a method which can examines virus pathogenic in gastroenteritis excrement specimen. Methods Choosing six positive specimens which tested in our laboratory, include adenovirus, ealicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification（SISPA） constructs a gene bank. Looks up the viral gene fragment in gene bank. Results Obtaining corresponding viral acid sequence in six specimens. Conclusion This research can examine enterovirus and the virus which cause diarrhea, It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.