脂肪组织内脂素、脂联素的表达与代谢综合征

The Association of the Adiponectin and Visfatin Expression in Adipose Tissues in Patients with Metabolic Syndrome

背景内脂素是最近发现的一种脂肪细胞因子,参与烟酰胺腺嘌呤二核苷酸(NAD)的合成,其表达受脂肪组织多少、分布、炎症状态等影响。大多数研究认为内脂素在高血压、糖尿病、动脉粥样硬化时增高,但也有报道是降低的。目的探讨代谢综合征(MS)患者脂肪组织内脂素、脂联素表达水平的变化及其与MS的关系。方法按照2005年国际糖尿病联盟(IDF)关于MS的诊断标准选取择期行胆石症手术的MS患者36例,非MS患者32例。测量身高、体质量、腰围、臀围后,留取血样检测空腹血糖、血脂、空腹胰岛素、血浆脂联素和内脂素等指标。采用半定量RT-PCR方法检测网膜与腹部皮下脂肪组织脂联素与内脂素的表达情况。结果MS组网膜脂肪组织脂联素mRNA的表达量(0.73±0.16)显著低于非MS组(0.83±0.17,P<0.05);进行性别分组后发现,男性MS组与非MS组比较,网膜脂肪组织脂联素mRNA表达差异有统计学意义(0.69±0.17比0.83±0.19,P<0.05)。内脂素mRNA的表达量在组间差异无统计学意义。血浆脂联素水平与HDL-C呈正相关(r=0.513,P<0.05),网膜脂肪组织内脂素mRNA表达量与HOMA-IR呈正相关(r=0.219,P<0.05),血浆内脂素与总胆固醇、三酰甘油、低密度脂蛋白胆固醇呈正相关。结论脂肪组织脂联素的表达水平变化可能与MS的发生有关,而内脂素在代谢综合征及非代谢综合征间没有差别。

Background is an adipose-derived adipocytokine involved in nicotinamide adenine dinucleotide （NAD） synthesis. The expression of visfatin was affected by the obesity, distribution of adipose tissue, and the inflammatory state, etc. However, visfatin level in hypertension, diabetes, and atheroselerosis was controversial. Objective To investigate the expression of the adiponectin and visfatin in fidipose tissues and its relationship with components of metabolic syndrome （MS）. Methods Thirty-six MS patients and 32 age and sex matched non-MS control subjects underwent operation due to gallstone were enrolled. Data on height, body weight, waist circumference（WC） and hip circumference （HC） were collected. Fasting plasma glucose {FPG）, triglyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol {HDL-C）, low density lipoprotein cholesterol（LDL-C）, fasting insulin, adiponectin and visfatin were determined. Expressions of adiponectin and visfatin in abdominal subcutaneous and omental adipose tissues were assessed by semi-quantitive RT-PCR. Results The adiponectin mRNA expression in omental adipose tissue was significantly lower in MS patients than that in control subjects （0.73±0.16 vs 0.83±0.17, P〈0. 05）. In male subjects, the level of adiponectin mRNA expression in ofnental adipose tissue was lower in MS group than that in non-MS group （0.69±0.17 vs 0. 83±0.19, P（0.05）. However, there is no significant difference of visfatin mRNA expression between two groups. Parti＇al correlation analysis showed that the level of adiponectin in blood plasma was positively correlated with HDL-C （r=0. 513, P（0.05）. The eXpression of visfatin mRNA in omental adipose tissue was positively correlated with HOMA-IR （r= 0. 219, P%0.05）, and the level of visfatin in blood plasma was positively correlated with TC, TG and LDL-C. Conclusion Adiponectin level in visceral fat tissue may be related with metabolic syndrome, while no differences in visfatin were found between pa- tients with and without metabolic syndrome.