Kank1基因在鼻咽癌中的表达及意义

The expression and significance of kankl gene in nasopharyngeal carcinoma

目的检测Kank1基因在鼻咽癌（NPC）细胞与组织中的表达，探讨其在NPC发病中的作用。方法采用半定量RT．PCR技术，分别检测4株NPC细胞（CNE1、CNE2、5—8F和6—10B）及永生化鼻咽上皮细胞株NP6972．22例NPC患者Kank1基因的表达，并与11例非癌对照组人群进行比较。结果Kank1基因在NPC细胞中表达降低，NPC患者Kank1的表达率显著低于对照组（P〈0．05）。结论Kank1基因表达可能在NPC发病机制中具有重要意义。

Objective To investigate the expression and significance of Kank1 gene in NPC occurrence. Methods The expressions of kank1 were detected in 4 NPC cell lines（ CNE1 ,CNE2,5 -8F and 6 - 10B）, NP69 cell hne, 22 eases of NPC and 11 cases of control patients using a quantitative nested polymerase chain reaction （RT-PCR）. Results The expressions of Kank1 in 4 NPC cell lines were lower than that of NP69 cell. The expressions of Kank1 gene in NPC patients were significantly lower than that of normal controis. Conclusion The Kank1 gene may play an important role in the mechanism of NPC occurrence.