四氧嘧啶剂量、给药途径、给药次数与小鼠体重对制作小鼠糖尿病模型的影响

Effect of different dosage and the way and times of administration of alloxan and the weight of mice on diabetic mouse model

目的探讨四氧嘧啶剂量、给药途径、次数和小鼠体重对四氧嘧啶致小鼠糖尿病模型的影响。方法使用四氧嘧啶根据不同给药途径、方式和小鼠体重对实验动物进行分组：观察四氧嘧啶剂量、给药途径、给药次数及小鼠体重对糖尿病小鼠血糖、体重、成模率和死亡率的影响。结果与生理盐水组相比，实验所用各造模法均可使血糖升高明显；腹腔注射一次法给药组血糖升高优于腹腔注射两次法给药组：一次法腹腔注射给药组和一次法静脉注射给药组血糖升高差异无统计学意义；腹腔注射一次法给药各组，中体重组的小鼠与高和低体重组小鼠比较，血糖升高显著；除腹腔注射两次法给药组外，各实验组糖尿病小鼠体重均有下降，并且腹腔注射一次法给药中体重组小鼠成模率为100％。结论用四氧嘧啶制作小鼠糖尿病模型，一次腹腔注射四氧嘧啶200mg／kg可提高糖尿病小鼠模型的成功率，且小鼠体重以18—22g为最佳。

Objective To explore the effects of the dose and the way and times of administration of alloxan （ALX） and body weight of mice in diabetic mice model. Methods To observe the effects of the dose, the way and times of administration of alloxan, and body weight of mice on serum glucose, body weight ,the incidence and mortality of diabetes in mice were compared. Result Compared with normal saline, all methods of copying model could significantly promote serum glucose. Serum glucose of one-step administration of intraperitoneal group was higher than two-steps group. One-step administration of intraperitoneal group had no statistics influence with one-step administration of intravenous group. The mice of middle weight group had higher serum glucose than high and low weight group. Body weight of mice was declined in all groups except two-steps administration intraperitoneal group, and the incidence of diabetes was 100 percent in one-step middle weight group. Conclusion One-step intraperitoneal administration of alloxan 200mg/kg could promote the incidence of diabetes models, and the mice weighting 18 -22g are the best.