宫颈癌放疗前后FHIT蛋白的表达及与放疗疗效的关系

The expression of FHIT protein in cervical carcinoma and the relationship between expression of FHIT and treatment effect of radiotherapy.

目的:探讨宫颈癌脆性组氨酸三联体基因(FHIT)蛋白表达与患者临床病理指标、放疗疗效及无瘤生存时间的关系。方法:用免疫组化法检测50例ⅡB～ⅢB期宫颈癌放疗前、DT10Gy,DT30Gy放疗中和放疗后FHIT蛋白的表达。结果:(1)放疗前50例患者FHIT蛋白表达阳性率36%,与病理分级,淋巴结转移有关,与年龄、临床分期、肿瘤大小无关;(2)放疗DT10Gy,DT30Gy和放疗结束后50例患者FHIT蛋白表达阳性率分别为56%,68%和84%,比放疗前明显升高(P<0.05);(3)放疗前FHIT蛋白阳性表达和阴性表达患者的放疗疗效分别为88.8%和59.4%,差异有统计学意义(P<0.05);(4)FHIT阳性表达无瘤生存时间与FHIT阴性表达无瘤生存时间无显著差异。结论:FHIT基因在宫颈癌发生发展中起重要作用,放疗可引起FHIT表达增加,FHIT蛋白的表达有助于判断宫颈癌患者放疗的敏感性。

Objective：To investigate the relationship between FHIT expression and the clinical pathological feature, treatment effect of radiotherapy, and the time of no disease in cervi- cal carcinoma. Method： Expression of FHIT protein was detected by immunohistochemical in 50 cases of squamous cell cervical carcinoma. Results： The positive rates of FHIT expression before radiotherapy DT10Gy, DT30Gy and after radiotherapy were 36% ,56% ,68% and 84% ,respec- tively（P〈0.05）. There were significant differences between FHIT expression and histological grades and lymph node metastasis （P〈0.05）. FHIT expression was not correlated with age, clinical stage and diameter of the turnout（P〉0.05）. There was a significant correlation between FHIT expression and treatment effect of radiotherapy（P〈0.05 ）. FHIT expression was not corre- lated with the time of no disease. Conclusion：FHIT may be very important in development of cervical carcinoma,radiotherapy can reinforce the expression of FHIT protein. FHIT expression may be helpful in judgment of the radiosensitivity of cervical carcinoma.