褐黄孢链霉菌生产纳他霉素工艺条件研究

Study of Cultivation Conditions for Natamycin Production by Streptomyces gilvosporeus

目前，国内纳他霉素产量低，为提高其产量，实现其工业化生产，主要对褐黄孢链霉菌ATCC13326发酵生产纳他霉素的培养条件及前体添加策略进行研究．得出最佳培养条件为：培养温度28℃，种龄40～44h，接种量10％，装液量10％，初始pH7．3，发酵24h时加入0．6％的前体丙酸钠．在此条件下摇瓶分批发酵生产纳他霉素的产量可达到6．87g／L，比未添加前体的纳他霉素产量（3．72g／L）提高了84．7％．利用10L发酵罐进行纳他霉素的发酵研究，在最优培养条件下，发酵罐中的纳他霉素产量可达到8．34g／L，比摇瓶分批发酵生产纳他霉素产量（6．87g／L）提高了21．4％．

In order to enhance the production of natamycin and realize industrial production, in this study, the cultivation conditions as well as the effect of precursor addition on the natamycin production by S. gilvosporeus ATCCI3326 were investigated. The optimizcd cultivation conditions were as follows：initial pH 7.3,age of inoculum 40~44 h,inoculum level 10 %, shake-flask working volume 30 mL/300 mL,temperature 28℃ ,cultivation time 120 h,and addition of 0.6% sodium proprionate into the broth after 24 h of cultivation. On the optimum conditions a natamycin production of 6.87 g/L could be reached in the shake flask culture after 120 h cultivation, which was 84.7% higher than that of cultivations without precursor addition. Even higher natamycin concentration （8.43 g/L） on the optimal cultivation conditions, which was 21.4 % higher than that could be expected in the shake-flask culture （6.87 g/L）.