去乙酰真菌环氧乙酯发酵液预处理工艺

Technology of Pretreating Fermentation Broth of Deacetylmycoepoxydiene

为了降低去乙酰真菌环氧乙酯发酵液粘度,改进过滤效果,考察了β-葡聚糖酶对去乙酰真菌环氧乙酯发酵液粘度的影响,并进一步研究了不同类型的絮凝剂对发酵液过滤速度、沉降率、有效过滤体积和去乙酰真菌环氧乙酯含量的影响.通过正交试验,确定了非离子型聚丙烯酰胺的最佳絮凝工艺条件,应用统计学手段获得了最佳酶促反应时间和酶用量.结果表明,β-葡聚糖酶和非离子型聚丙烯酰胺的联合使用,可使滤速提高80%,有效过滤体积增加50%,保证了去乙酰真菌环氧乙酯的发酵量.

In order to lower the viscosity of deacetylmycoepoxydiene fermentation broth and improve the effect of filtering, the impact of β-glucanase on the viscosity of DAM fermentation broth was studied. The effects of different types of flocculants on filtration rate of fermentation-broth, sedimentation rate, the effective filter volume and concentration of DAM were further studied. Through orthogonal test, the optimal flocculation conditions of the non-ionic flocculants were obtained, and enzymatic reaction time and enzyme dosage of β-glucanase were determined by statistical methods. The results show that the filtration rate is increased by 80% and effective filter volume is increased by 50% through the joint use of the β-glucanase and non-ionic flocculants. It ensures the fermentation volume of DAM.