放线菌HJ1-2菌株发酵液抑菌谱及稳定性的研究

Study of Antimicrobial Spectrum and Stability of the Fermentation Broths Produced by Actinomycete Strain HJ1-2

采用菌丝生长速率法、管碟法和活体组织法测定发酵液的抑菌活性,在不同条件下测定发酵液的稳定性,捷克溶剂系统纸层析法鉴别发酵液中抗生素类型。发酵液的抑菌活性测定结果表明,放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用,其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上;供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强,抑菌圈直径为17.75 mm;放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100%和71.39%。发酵液的稳定性测定结果表明,发酵液对热、酸和紫外线均较稳定,发酵液在80℃处理后抑菌活性开始下降,发酵液在pH3~9条件下抑菌活性稳定。捷克溶剂系统纸层析法测定表明,发酵液中主要抑菌活性物质为大环内酯类抗生素。该菌株的代谢产物具有广谱抑菌活性和较强的稳定性,具有一定开发价值。

By means of mycelium growth rate,cup-plate method and organize method,the antimicrobial activity of bioassay was studied.The stability of the fermentation broths in different conditions was measured.By comparing with the curves of classical antibiotics in Doskochilova system,the antibiotic type of fermentation broths was identified.The antimicrobial tests showed,the inhibition rate of all the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times.It showed strongest inhibition effect against Erwinia carotorora（17.75 mm）.Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%,respectively.Through the research of stability of the fermentation broths of actinomycete strain HJ1-2 to heat,acid,alkali and light,it had a stable activity to heat,acid and UV,but unstable to alkali.The active ingredients in the fermentation broths were identified as macrolides antibiotics.Antimicrobial spectrum of actinomycete strain HJ1-2 was widely.Antimicrobial activity of the fermentation broths was stable.The actinomycete strain HJ1-2 was worth developing.