食源性大肠杆菌耐药性检测

The Antibiotic Resistance of Food-borne Escherichia coli

采用美国临床实验室标准化委员会(National Committee of Clinical Laboratory Standard简称NC-CLS)推荐的琼脂稀释法,以大肠埃希氏菌ATCC25922、粪肠球菌ATCC29212和金黄色葡萄球菌ATCC29213为质控菌株,对411株分离自鸡肉和凉拌菜的大肠杆菌进行了6大类共12种抗生素的药敏性检测。结果表明受试菌株对四环素的耐药率(98.3%)最高,其次是链霉素(71.3%)、奈定酮酸(68.4%)、阿莫西林(67.6%)、氨苄青霉素(61.8%)、环丙沙星(50.6%)、氯霉素(48.4%)、卡那霉素(40.6%)、庆大霉素(36.7%),所有受试菌株对阿米卡星的敏感性最强,仅有11.9%(49)的耐药率,其次是头孢西丁(14.1%)和头孢哌酮(29.9%)。247(60.1%)株分离菌表现5重以上耐药性,其中鸡肉分离株占236株。

411 Escherichia.coli（E.coli） isolates recovered from retail meats and Chinese salads that collected in supermarkets and free markets in Xi＇an and Yangling areas of Shaanxi Province were studied to determine antibiotic susceptibility.Antimicrobial susceptibility to 12 antibiotics of 411 E.coli isolates were determined by using agar dilution method,which was recommended by National Committee of Clinical Laboratory Standard（NCCLS）,and E.coli ATCC25922,E.faecalis ATCC29212 and S.aureus ATCC29213 as standard control strains.Results showed that 98.3% of the E.coli isolates were resistant to tetracycline,followed by resistance to Streptomycin（71.3%）,Nalidixic acid（68.4%）,Amoxicillin（67.6%）,Ampicillin（61.8%）,Ciprofloxacin（50.6%）,chloramphenicol（48.4%）,Kanamycin（40.6%）,gentamicin（36.7%）.The most sensitive antibiotical is Amikacin（88.1%）,followed by Cefoxitin（85.9%） and Cefoperazone（70.1%）respectively.247 isolates（60.1%） were multidrug resistant（MDR） strains（more than 5）,236 isolated from chicken.