气相色谱内标法测定深海鱼油中的EPA和DHA

Determination of EPA and DHA in Deep Sea Fish Oil by Gas Chromatography using Internal Standard Method

用氢氧化钾—甲醇酯化法将深海鱼油中的脂肪酸快速、有效地转化成脂肪酸甲酯,用毛细管气相色谱法进行测定,以二十三碳酸脂肪酸甲酯为内标,建立EPA和DHA的定量分析方法。该法对EPA、DHA的相对标准偏差分别为3.6%和3.2%,回收率分别为99.3%和101.6%。

Fatty acids in deep sea fish oils were methylated with KOH-CH3OH solution. The obtained fatty acid methyl esters were determined by gas chromatograph and quantified by internal standard C23：0 methyl ester. The RSD values of the method for the determination of EPA and DHA were 3.6% and 3.2%, respectively. And the recoveries of EPA and DHA were 99.3% and 101.6%, respectively.