氨氯地平对CHF大鼠血浆ET-1和BNP表达的影响

Effect of Amlodipine on Expression of ET-1 and BNP in Plasma of Rats with Congestive Heart Failure

目的探讨氨氯地平对充血性心力衰竭(CHF)大鼠血浆中内皮素-1(ET-1)和B型钠尿肽(BNP)表达的影响。方法SD大鼠随机分为3组:对照组、心衰组、氨氯地平组。CHF组大鼠腹腔注射阿霉素累积剂量达20mg/kgBW制作CHF模型,氨氯地平组大鼠腹腔注射阿霉素同时每天给予氨氯地平10mg/kg。4周后各组大鼠经颈内动脉插管至左心室行血流动力学测定,同时测定大鼠血浆中ET-1和BNP的含量。结果CHF组大鼠左室压力最大上升速率(+dp/dtmax)和最大下降速率(-dp/dtmax)均显著低于对照组,其血浆内ET-1和BNP均显著高于对照组(P<0.01)。氨氯地平组±dp/dtmax均显著低于对照组但高于CHF组,其血浆内ET-1和BNP均显著高于对照组低于CHF组(P<0.01)。结论氨氯地平治疗CHF大鼠的作用机制之一可能是通过保护CHF大鼠左室功能、结构及心肌细胞,间接降低ET-1、BNP表达水平。

Objective To explore the effect of amlodipine on the expression of ET-1 and BNP in plasma of rats with congestive heart failure. Methods Rats were diveded into three groups, control, chronic heart failure and amlodipine therapy group. Chronic heart failure was induced with adriamycin 20 mg·kg-1 BW. Rats in amlodipine group received amlodipine 10 mg·kg-1·d-1 and adriamycin at the same time. Hemodynamic measurement was carried out after 4 weeks and the contents of ET-1 and BNP in plasma of each group were detected. Results Compared with control group, the ± dp/dtmax in chronic heart failure group significantly decreased, and the expression of ET-1 and BNP in plasma significantly increased （P〈0.01）. The ±dp/dtmax in amlodipine group was significantly lower than that in control but obviously higher than that in chronic heart failure group, and the expression of ET1 and BNP in plasma was significantly higher than that in control but obviously lower than that in chronic heart failure group （P〈0.01）.Conclusions One mechanism of amlodipine treatment of rats with congestive heart failure may be indirectly reduced the expression of ET-1 and BNP through maintaining the function and structure of left ventricular and protecting cardiac cells.