甘蓝显性雄性不育基因CDMs399-3紧密连锁的分子标记

Molecular Markers Closely Linked to the Dominant Male Sterility Gene CDMs399-3 in Brassica oleracea

【目的】筛选与甘蓝显性雄性不育基因CDMs399-3紧密连锁的分子标记。【方法】以甘蓝显性核不育为不育源转育获得的芥蓝BC4分离群体226个单株为试材,利用SRAP、SSR技术结合集群分析法筛选与CDMs399-3紧密连锁的分子标记。【结果】获得8个SRAP、1个SSR和1个SCAR标记与不育基因连锁,最近的双侧翼标记ENA14F-CoEm7R和8C0909,连锁距离分别为0.53和2.55cM,并将侧翼标记ENA14F-CoEm7R转化为SCAR标记。【结论】本研究丰富了与CDMs399-3连锁的分子标记,获得多个紧密连锁的标记为该不育基因标记辅助转育和进一步精细定位奠定了良好的基础。

[Objective] In this study, molecular markers linked to CDMs399-3 gene in Brassica oleracea were screened. [Method] SRAP and SSR technologies were used to identify markers linked to CDMs399-3 based on BSA method. The bulks were formed using malesterile and malefertile individuals from a segregating BC4 population. [ Result ] A total of 8 SRAP were identified, 1 SSR and 1 SCAR markers closely linked to the male sterility gene. The two flanking markers, ENA14F-CoEm7R and 8C0909, were linked to the CDMs399-3 with a distance of 0.53 cM and 2.55 cM, respectively. ENA14F-CoEm7R was converted into a sequence characterized amplified region （SCAR） marker. [Conclusion] The markers developed here founded a solid basis for fining mapping of CDMs399-3 gene and were useful for accurate marker-assisted selection （MAS） of the male sterility gene.