摘要
采用CTAB法提取甜菜基因组DNA,对不同作物(甜菜、苜蓿、玉米)的SSR引物进行了筛选。每对引物都有其最适宜的退火温度(Tm),Tm的高低对扩增结果有较大影响。该实验主要通过改变PCR扩增的退火温度(当退火温度比较低时,SSR-PCR扩增的效果条带较多,特异性不好;当退火温度比较高时扩增的条带较少,而且条带也较弱,扩增效率不高)选择合适的引物。从不同作物查阅的91对SSR引物中共筛选出带纹清晰的引物7对,作为甜菜遗传多样性的SSR分析引物,为进一步进行甜菜的分子育种提供理论依据。
The total DNAs of beet were extracted by CTAB method. 7 primers were screened from 91 primers by changing the annealing temperature for SSR-PCR reaction system. These primers amplified distinct bands, and their polymorphism is also very high. These polymorphic primers could be further used in beet molecular geneticbreed.
出处
《中国糖料》
2009年第4期6-8,共3页
Sugar Crops of China
基金
国家甜菜现代产业技术体系建设项目(2060302)资助
