青天葵具抗肿瘤活性石油醚部位的成分研究

Studies on chemical constitutents of petroleum ether extract with anti-tumor activity from Nerviliae fordii

通过体外抑制肿瘤细胞生长活性筛选确定青天葵95%乙醇(体积分数)提取物及其石油醚萃取部位为抗肿瘤活性部位.综合利用多种色谱手段及重结晶方法从石油醚部位分离得到了8个化合物,并运用理化性质和波谱技术分析确定了化合物结构.其结构分别为:环桉烯醇(cycloeucalenol)(1);豆甾醇(2);谷甾醇(3);熊果酸(4);aurantiamide acetate(5);(20S,22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol(6);6-methoxy-cerevisterol(7);和β-胡萝卜苷(8).其中,化合物3、5、6、7和8均为首次从该植物中分离得到.

The results of the in vitro antitumor activity screenings indicated that 95% ethanol extract and the petroleum ether fraction from the extract showed a significant inhibitory activities on growth of cancer cells. Eight compounds were isolated from this fraction by a combination of various chromatography methods and re-crystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data, and were identified as cycloeucalenol （1） ; stigmaterol （2） ; sitosterol （3） ; ursolic acid （4） ; aurantiamide acetate （5） ; （20S, 22E, 24R）-ergosta-7,22-dien-3β,5α,6β-triol （6） ; 6-methoxy-cerevisterol （7）; are obtained from the plant for the and fl-daucosterol （8）. Among these compounds, 3, 5, 6, 7 and 8 first time.