小西葫芦黄花叶病毒山东南瓜分离物的分子特性

Molecular characterization of Zucchini yellow mosaic virus isolate infecting pumpkin in Shandong

Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence,840 bp of cp gene and 211 bp of 3′-untranslated region of the isolate ZYMV-Liaocheng was determined.The cp gene of ZYMV-Liaocheng shared identities of 81.4%-98.8% and 89.4%-99.5% at nucleotide and amino acid levels,respectively,with other ZYMV sequences available in the GenBank.Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes.ZYMV-Liaocheng belonged to genotype I,which contained isolates from Asia,Europe and America.Genotypes III and V were unique and contained only isolates from East Asia.The isolates from East Asia had the highest variability.

Zucchini yellow mosaic virus （ZYMV） was detected by RT-PCR from pumpkin （ Cucurbita moschata） plant showing yellowing and mosaic symptom from Liaocheng, Shandong Province. The 3＇-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence, 840 bp of cp gene and 211 bp of 3＇- untranslated region of the isolate ZYMV-Liaocheng was determined. The cp gene of ZYMV-Liaocheng shared identities of 81.4% - 98.8% and 89.4% - 99.5% at nucleotide and amino acid levels, respectively, with other ZYMV sequences available in the GenBank. Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes. ZYMV-Liaocheng belonged to genotypeⅠ, which contained isolates from Asia, Europe and America. Genotypes Ⅲ and Ⅴ were unique and contained only isolates from East Asia. The isolates from East Asia had the highest variability.