响应面法优化桑黄菌产胞外多糖液体发酵培养基的研究

Study on the Optimization of Liquid Fermentation Culture Medium for Producing Phellinus Linteus Expolysaccharide by Response Surface Methodology

利用响应面分析法对对桑黄菌产胞外多糖的液体发酵培养基进行了优化。研究碳源、氮源、无机盐和维生素对桑黄菌丝及胞外多糖产量的影响。在单因素筛选实验的基础上,利用Box-Benhnken设计和响应面分析法对碳源、氮源、无机盐和维生素进行分析。结果表明,桑黄菌产胞外多糖的液体发酵培养基最佳组合为:糙米粉为43.608 g/L,豆饼粉为4.251 g/L,KH_2PO_4为3.251 g/L,V_(B2)为325.531μg/L。在此条件下的验证试验表明,胞外多糖平均可达3.088 g/L。

Liquid fermentation culture medium for producing phellinus linteus expolysaccharide was optimized by response surface methodology. The effects of carbon source, nitrogen source, metallic ions, vitamin on the yield of mycelium and expolysaccharide were studied. Based on single factor experiments, the concentration of carbon source, nitrogensource, metallic ions and vitamin was analyzed by Box-Benhnken design and response surface methodology. The results showed that the optimal culture medium was as follows： 43.608 g/L brown rice powder, 4.251 g/L soybean powder, 3.251 g/L KH2PO4, 325.531 μg/L VB2. The average yield of expolysaccharide reached 3.088 g/L after validated fermentation experiment under optimal condition.