刺五加有效部位对C_(57)BL/6小鼠PD模型GABA、TH、GFAP影响的实验研究

Impact of the effective parts of Acanthopanax senticosus on GABA,TH and GFAP in C_(57) BL/6 mouse model of parkinson's disease

目的:研究刺五加有效部位对C57BL/6小鼠的帕金森病(PD)模型γ-氨基丁酸(GABA)、黑质酪氨酸羟化酶(TH)、及海马区胶质原酸性蛋白(GFAP)的影响,初步探讨其治疗帕金森病的作用机制。方法:用MPTP对C57BL/6小鼠制备PD模型,总量为150mg/kg。连续给予刺五加有效部位20 d,测定小鼠的爬杆时间,用免疫组化法测定小鼠黑质纹状体GABA、TH及海马区GFAP的阳性表达。结果:20 d后,爬杆时间、GABA、TH给药组相对模型组P<0.05。而GFAP没有出现显著差异。结论:刺五加有效部位可以增强GABA和TH的阳性表达,而对海马区GFAP则无影响,推测其对MPTP导致的PD可能有脑保护作用。

AIM： To study the effect of effective parts of Acanthopanax senticosus on Parkinson＇ s disease mouse model （from C57 BL,/6 strain ） concerning Gamma-amino butyric acid （GABA）, nigra-striatum tyrosine hydrozylase （TH） and hippocampal glial fibrillary acidic protein （GFAP）. METHODS： In MFFP （1-methyl-4- phenyl-1,2 ,3 ,6-tetrahydropytidine） -induced PD mouse model （accumulative dose amounted to 150 mg,/kg） poleclimbing time was determined of mice after receiving effective parts of Acanthopanax senticosus for 20 d, and GARA, TH and GFAP expression were assayed simultaneously with the help of immunohistochemical method. RESULTS： Mice＇s pole-climbing time was measured, GABA and TH made a significant difference between treated group and control group （ P 〈 0.05 ） , There was no significant difference in. CONCLUSION ： Based on enhancement of GABA and TH expression derived from the effective parts of Acanthopanax senticosus （ except for GFAP）, we speculate that the effective parts could protect the brain injury in mouse from Parkinson＇ s disease induced by MPTP.