RP-HPLC同时测定清肺化痰丸中3种成分含量

Determination of three components in Qingfei Huatan Pill by RP-HPLC

目的：采用RP-HPLC法测定清肺化痰丸（胆南星,枳壳,橙皮,黄芩等）中柚皮苷、橙皮苷和黄芩苷的含量。方法：采用十八烷基硅烷键合硅胶（200 mm×4.6 mm,5μm）为固定相,以乙腈-水（20∶80）（用磷酸调节pH值至2.2）为流动相,在280 nm波长测定柚皮苷、橙皮苷和黄芩苷。结果：柚皮苷在1.24-6.2μg范围内线性关系良好（r=0.999 7）,回收率为99.29%（RSD为1.78%）。橙皮苷在1.1-5.5μg范围内线性关系良好（r=0.999 8）,回收率为100.08%（RSD为1.44%）。黄芩苷在2.32-11.6μg范围内线性关系良好（r=0.999 9）,回收率为98.76%（RSD为1.42%）。结论：该方法中简便准确,重复性好,可作为本产品的质量控制方法。

AIM： To establish a RP-HPLC for determing the contents of naringin, hesperidin and baicalin in Qingfei Huatan Pill （Arisaema Cumbile, Fructus Aurantii, Pericarpium Citrireticulatae, Radix Scutellariae, etc. ）. METHODS： Naringin, hesperidin and baicalin were separated on a RP C18 column. Acetonitrile-water （20：80） （ adjusted to pH 2.2 with phosphoric acid） was used as the mobile phase. The detective wavelength was set at 280 nm. RESULTS ： The linear ranges were in 1.24 - 6.2 p.g for naringin （ r = 0.999 7 ）, 1.1 - 5.5 μg for hesperidin （r =0. 999 8） and 2.32 - 11.6 p.g for baicalin（r =0.999 9）. The recoveries were 99.29% with RSD of 1.78% for naringin,100.08% with RSD of 1.44% for hesperidin and 98.76% with RSD of 1.42% for baicalin respectively. CONCLUSION： The method is simple, accurate and reproducible. It may well be used for the quality control of this product.