纳洛酮对小鼠海马CA1区神经元缺血再灌注损伤干预效果的观察

Effect observation of naloxone intervention after reperfusion on cerebral ischemia-reperfusion injury to neurons in mice hippocampal CA1

目的进一步验证脑缺血再灌注损伤实验动物模型的可行性,初步探讨纳洛酮再灌注后干预对脑缺血再灌注损伤的影响,探索脑缺血再灌注损伤时纳洛酮后干预的最佳时机。方法采用结扎双侧颈总动脉小鼠脑缺血再灌注损伤模型,分别观察纳洛酮再灌注后干预对海马CA1区神经元存活细胞数和细胞形态的影响。结果纳洛酮治疗组小鼠海马CA1区存活细胞数明显高于缺血再灌注对照组,差异有统计学意义(P<0.05),变性细胞率明显低于缺血再灌注对照组,差异有统计学意义(P<0.05),而与假手术组比较差异无统计学意义(P>0.05),纳洛酮治疗组各组内比较差异无统计学意义。纳洛酮各组与缺血再灌注对照组比较变性坏死细胞明显减少,细胞形态接近正常,间质水肿得到改善。结论从形态学的角度,纳洛酮后干预确实能减轻脑缺血再灌注损伤,在1.5h内不同时间给予纳洛酮的干预效果没有明显差别。

Objective To validate the experimental animal model for cerebral ischemia-reperfusion injury, and to investigate the effects and optimal timing of naloxone intervention after reperfusion on cerebral ischemia-reperfusion injury. Methods Mice model of cerebral ischemia reperfusion injury was established with bilateral ligation of common cervical arteries. The effect of naloxone intervention after reperfusion on viable cell counts and cellular morphology in neurons of hippocampal CA1 was observed. Results The naloxone group presented with higher viable cell counts （P〈 0.05） and lower percentage of degenerated neurons （P〈0.05） at hippocampal CA1 as compared with the normal saline group. Yet these findings were not shown when comparisons were made between naloxone group and sham-operation group or between sub-groups treated with naloxone. Use of naloxone was shown to result in less degenerated or necrotized cells, almost normal morphology and improved edema of the matrix. Conclusion The naloxone administration after reperfusion may morphologically allow for mitigation of injury to the neurous of mice hippocampal CA1. Efficacy appeared comparable when naloxone was started somewhere within 1.5 hours from resperfusion ischemia injury.