转基因pYBGA1酵母发酵木质纤维素水解液制备燃料乙醇

Ethanol production from lignocellulose hydrolysate by yeast-pYBGA1

采用转基因pYBGA1酵母发酵葡萄糖及纤维二糖培养基,通过交叉实验研究了温度、酵母投入量对乙醇产率的影响;对木质纤维素进行爆破预处理,得到葡萄糖和纤维二糖的质量分数占50%预处理水解液,采用转基因pYBGA1酵母对预处理水解液发酵制备乙醇。结果表明,转基因pYBGA1酵母最佳发酵温度为28℃,最佳酵母投入量为108mL-1;当水解液中糖浓度为20 g/L时,预处理水解液发酵得到乙醇的最大浓度可达6.8 g/L。

he glucose and cellobiose was fermented by using pYBGA1 recombinant yeast, study the effects of the temperature and concentration of yeast-pYBGA1 on the ferment ethanol rate by using cross experiments; the lignocellulosic was pretreated through steam explosion pretreatment method, the glucose and cellobiose content in the pretreatment hydrolysate was 50 % ; the pretreatment hydrolysate was fermented by using yeast-pYBGA1 to prepare the ethanol. Results shows that, the best fermentation temperature was 28℃ and the best to the ethanol productivity was 10^8 mL^- 1 ; the highest concentration of ethanol by direct fermentation of mixed sugar in lignocellulose-pretreatment hydrolysate is 6.8 g/L when the suger concentration is 20 g/L in the hydrolysate.