石头菇母种培养基优化研究

Study of Mushroom Stone Optimization Culture Medium

利用3因素5水平二次通用旋转组合设计,优选适合石头菇母种的培养剂,研究了葡萄糖、蛋白胨、硫酸镁、磷酸二氢钾对石头菇菌丝的长速、干重的影响。结果表明：该试验条件下葡萄糖、蛋白胨、无机盐（硫酸镁、磷酸二氢钾）3因素中,对石头菇菌丝的长速、干重的效应为葡萄糖〉无机盐〉蛋白胨。获得石头菇菌丝的长速〉0.44 cm/d,菌丝干重〉27.86 mg/总量,用葡萄糖19.866～21.216 g/L,蛋白胨4.662～4.806 g/L,硫酸镁、磷酸二氢钾5.164～5.948 g/L。

Used three common factors in the second five-level rotation design,to selecting for the cultivation species of mushrooms stone home agent,studied glucose,peptone,magnesium sulfate,potassium dihydrogen phosphate rock deliciosus of wire-speed and long,dry weight impact.The results showed that under the experimental conditions of glucose,peptone,inorganic salts（magnesium sulfate,potassium dihydrogen phosphate） three factors,to silk long stone deliciosus speed, the effeet of dry weight for glueose〉salts〉peptone. Access to the stones of the long wire-speed deliciosus） 0. 44 cm/day, dry weight of mycelium〉27. 86 mg/total glucose 19. 866-21. 216 g/L, peptone 4. 662-4. 806 g/L, magnesium sulfate, potassium dihydrogen phosphate were 5. 164-5. 948 g/L.