特发性矮小症患儿生长激素结合蛋白测定的意义

Significance of Serum Growth Hormone-Binding Protein Detection in Children with Idiopathic Short Stature

目的探讨特发性矮小(ISS)患儿血清生长激素结合蛋白(GHBP)水平变化的意义。方法选择2006年9月-2008年4月于河北医科大学第三医院儿科门诊及住院ISS患儿30例为试验组,健康体检儿童30例作为健康对照组。采用酶联免疫吸附试验(ELISA)测定各组血清GHBP水平。结果ISS组患儿血清GHBP水平为(3261.0±646.3)μg/L,健康对照组儿童血清GHBP水平为(2026.0±497.2)μg/L,二组比较差异显著(t=-20.67P<0.01)。ISS组男童血清GHBP水平为(3196.4±670.6)μg/L,健康对照组男童血清GHBP水平为(1937.3±469.1)μg/L,二组比较有显著差异(t=-16.24P=0);ISS组女童血清GHBP水平为(3438.8±576.1)μg/L,健康对照组女童血清GHBP水平为(2270.0±520.6)μg/L,二组比较有显著统计学差异(t=-16.42P=0)。结论ISS患儿血清中增高的GHBP可能通过抑制了生长激素与靶组织受体的充分结合,从而导致生长激素不能发挥正常生理效应。

Objective To explore the change of growth hormone binding protein （GHBP） in children with idiopathic short stature （ISS）. Methods Thirty children with ISS from the pediatric department in the Third Hospital of Hebei Medical University and 30 age - and sex - matched normal - stature children were selected from Sep. 2006 to Jun. 2008. The concentration of GHBP was measured by enzyme linked immunosordent assay. Results Serum GHBP levels in ISS subjects was （3 261.0 ±646.3 ） μg/L, while the serum GHBP levels in normal control group was （ 2 026.0±497.2 ） μg/L, and there was statistically significant difference between both groups （ t = - 20.67 P 〈 0.01）. For the boys, the serum GHBP level in ISS group [ （3 196.4 ± 670.6） μg/L] was higher than that in normal control group [ （ 1 937.3± 469.1 ） μg/L] （ t = - 16.24 P = 0）. The girls＇ serum GHBP level in ISS subjects was （ 3 438.8 ± 576.1 ） μg/L, while their serum GHBP levels in normal control group was （2 270.0 ± 520.6 ） μg/L, and there was statistically significant difference between both groups （t = - 16.42 P = 0 ）. Conclusions Elevated serum GHBP levels in ISS children might inhibit the growth hormone receptor from fully integrating with the target tissue ,resulting in normal GH prevention from exerting physiological effects.