ACE基因I/D多态性与原发性高血压病中医证型的相关性研究

Correlativity Between ACE Gene I/D Polymorphism and TCM Syndromes of Primary Hypertension

目的观察原发性高血压病患者ACE基因I/D多态性的分布及与高血压病中医辨证分型的关系,为中医辨证论治提供客观依据。方法将472例原发性高血压病患者采用临床问卷调查,填写高血压病患者登记表格及中医症候分级量化表后进行辨证分型,分为无症状者、肝火亢盛型、阴虚阳亢型、痰湿壅盛型、阴阳两虚型、证型不明确者,分析其证型分布规律性。采空腹血6ml,分离白细胞,用苯酚-氯仿法从白细胞中抽提DNA。使用聚合酶链反应、琼脂糖电泳法进行ACE I/D基因分型。结果各证型组在各基因型之间均未见显著差异。但肝火亢盛、痰湿壅盛属于中医实证证型组的DD基因型与D等位基因的频率均明显低于其他属于虚证或虚实夹杂的证型组。结论ACE不同基因型分布对中医辨证分型可能没有影响。

Objective To observe the distribution of ACE Gene I/D polymorphism and its relationship with TCM syndromes of primary hypertension. Methods 472 cases of primary hypertension were surveyed with clinical questionnaire, registration form and syndrome quantified grading form, and then classified into asymptomatic syndrome, liver-fire hyperactivity syndrome, yin-deficiency and yang-hyperactivity syndrome, sputum-dampness retention syndrome, yin-yang deficiency syndrome, and uncertain syndrome. Six mililiter fasting blood was drawn from the patients and leukocytes were isolated, from which DNA was extracted with chloroform-phenol method, ACE I/D genotyping was examined with PCR method and gel electrophoresis method. Results There was no difference in genotyping among different TCM syndromes. However, DD genotype and D allele were less frequent in liver-fire hyperactivity syndrome and sputum-dampness retention syndrome. When we combined the two syndromes and compared it with other syndromes, there was a significant difference in the frequency of DD genotype and D allele （P 〈 0.01 ）. Conclusion The distribution of ACE Gene I/D polymorphism is correlative with TCM syndromes of primary hypertension.