趋化素样因子1在人动脉粥样硬化斑块组织中表达的研究

The expression of chemokine-like factor 1 in human atherosclerotic plaques

目的研究人动脉粥样硬化斑块组织和正常动脉壁中趋化素样因子1（CKLF1）mRNA及蛋白水平的表达的差异。方法通过实时定量PCR技术检测24例下肢动脉硬化闭塞症患者髂股动脉斑块标本（实验组）和25例尸体器官移植供体正常髂股动脉（对照组）标本中CKLF1mRNA水平的表达；通过连续薄层切片免疫组织化学染色技术检测17例实验组标本和10例对照组标本中CKLF1蛋白水平的表达。结果动脉粥样硬化斑块组织中CKLF1阳性表达的细胞类型有内皮细胞、平滑肌细胞和巨噬细胞。CKLF1mRNA在对照组中存在基础表达，相对量为2．4，在实验组中表达明显上调（相对量为132．2，P〈0．05）；CKLF1蛋白在实验组标本平滑肌细胞中的阳性表达率为100％，在对照组中为70％，表达明显上调（P〈0．01）。结论CKLF1在动脉粥样硬化斑块组织中表达显著上调，可能在动脉粥样硬化的免疫和炎症反应过程中起重要作用。

Objective To evaluate the difference of the expression level of chemokine-like factor 1 （ CKLF1 ） mRNA and protein between human atherosclerotie plaques and normal arteries. Methods Realtime quantitative PCR was performed with experimental group （24 cases of atherosclerotic plaques ） specimens and control group （25 cases of normal arteries） specimens. Serial microscopic sections were used for immunohistochcmistry with CKLF1 antibody in 17 experimental group specimens and 10 control group specimens. Results The endothelial cell, vascular smooth muscle cell （VSMC） and macrophage were CKLF1 positive expression cells in atheroselerotic plaques. There was a foundational expression of CKLF1 mRNA in control group （RQMedian = 2.4 ）, and the expression of CKLF1 mRNA in experimental group is upregulated significantly （ RQMedian = 132. 2, P 〈 0. 05 ） . The VSMC positive expression rate of CKLF1 protein was 100% in experimental group and 70% in control group, the former was up-regulated significantly. Conclusions The expression of CKLF1 is up-regulated in atherosclerotic plaques. It may play an important role in the process of immune and inflammatory responses of atherogenesis.