肺癌MMP-9、TIMP-1表达与微血管密度的关系及意义

The relationship of MMP-9 and TIMP-1 expression with microvascular density in lung carcinoma and its significance

目的探讨肺癌组织中MMP-9和TIMP-1的表达与肺癌血管生成的关系及其意义。方法采用免疫组化SP法检测84例肺癌组织中MMP-9、TIMP-1和CD34的表达。结果肺癌组织MMP-9表达显著高于癌旁正常肺组织（PP〈0.01）,小细胞肺癌和腺癌显著高于鳞癌（PP〈0.01）。肺癌MMP-9表达：有淋巴结转移者高于无淋巴结转移者（PP〈0.01）,Ⅲ-Ⅳ期高于Ⅰ-Ⅱ期（PP〈0.05）。肺癌组织TIMP-1表达显著高于癌旁正常肺组织（PP〈0.01）。肺癌MMP-9表达阳性者微血管密度高于MMP-9表达阴性者（PP〈0.01）。结论MMP-9促进肺癌血管生成,与肺癌侵袭转移密切相关。

Purpose To investigate the relationship of MMP-9 and TIMP-1 expression with angiogenesis in lung carcinoma tissue and its significance. Methods The expression of MMP-9 ,TIMP-1 and CD34 was detected in 84 lung carcinoma tissues by immunohistochemical method. Results The expression of MMP-9 in lung carcinoma tissues was significantly higher than that that in para-cancerous normal lung tissues （P 〈 0. 01 ）. Small cell lung cancer and adenocarcinoma had higher MMP-9 expression than squamous cell carcinoma （P 〈0.01 ）. The expression of MMP-9 was significantly higher in lung carcinomas with lymph node metastasis than in lung carcinomas without lymph node metastasis（P 〈 0. 01 ）, was significantly higher in stage- Ⅲ and-Ⅳ tumours than in stage-Ⅰ and- Ⅱ tumours（ P 〈 0. 05）. The expression of TIMP-1 in lung carcinoma tissues was significantly higher than that in para-cancerous normal lung tissues （P 〈 0. 01 ）. MVD was significantly higher in lung carcinomas with MMP-9 expression positive than in lung carcinomas with MMP-9 expression negative（P 〈 0. 01 ）. Conclusions MMP-9 can improve the neovascularization of lung carcinoma. MMP-9 expression is closely correlated with invasion and metastasis of lung carcinoma.