人白介素22对刀豆蛋白A致小鼠急性肝损伤的保护作用

The Protective Effects of Interleukin-22 on ConA-induced Acute Liver Injure in Mice

目的建立小鼠急性肝损伤模型,分析和评价人白介素22在小鼠急性肝损伤模型中的保护作用。方法利用刀豆蛋白A致小鼠急性肝损伤模型,分析并评价模型效果。在此基础上将雌性小鼠随机分为正常组、保护组及模型组;保护组和模型组分别尾静脉注射重组IL-22蛋白和生理盐水6h后,尾静脉注射ConA致小鼠急性肝损伤,正常组尾静脉注射等量生理盐水。16h后检测小鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)活性,同时取小鼠肝组织进行病理学检查。结果IL-22能明显降低小鼠血清的ALT、AST水平,减轻ConA对肝组织的病理损伤。结论IL-22对ConA致小鼠肝损伤具有保护作用,为进一步研究其保护作用机制奠定了基础。

Objective To establish a murine model of acute liver injure and evaluate the protective effects of interleukin - 22 in the model. Methods The animal model of acute liver injury was established by intravenous injection of ConA in BALb/C mice to assess the protective role of hIL -22. BALb/C mice were randomly divided into normal, protective and model group, which respectively received in- travenous injection of IL - 22 and saline. After 6h, protective and model group were received ConA by intravenous injection while normal group received only saline by intravenous injection. Serum transaminases （ALT, AST） activities of the protective and model group were assayed after 16h and 30h by obtaining blood from ophthalmic vein of mice. At the same time, the pathological changes were then ob- served by taking liver tissue of mice. Results Pretreatment with hIL - 22 before ConA injection could significantly decrease the elevation of ALT and AST. Pathohistology indicated the lesion of liver was markedly alleviated. Conclusion IL - 22 has protective effects on acute liver injure induced by ConA which lays foundation for futher studying its protective mechanism.