玻璃基底上的蛋白质微接触压印图型化(英文)

Using Microcontact Printing to Pattern the Proteins on Glass Surface

研究了一种将胶原Ⅰ型蛋白通过微接触压印技术图型化于玻璃基底表面的方法.采用标准光刻工艺制备印章母版,并运用反应离子刻蚀设备对印章表面进行氧等离子体处理,以期改善印章表面亲水性能.将涂敷了胶原Ⅰ型蛋白,并经返潮处理的印章以50 g/cm2大小的力与玻璃表面接触10 s,得到蛋白质微图型.结果表明,采用反应离子刻蚀技术能显著改善聚二甲基硅氧烷(PDMS)印章表面的亲水性.表面亲水性得到改善的PDMS印章,在经过湿盒返潮后,再进行微接触压印得到的蛋白质微图型其质量得到显著提高.

A method for patterning Col-I protein solutions onto glass surfaces with mirco contact printing has been discussed. The stamp master was fabricated with traditional photolithography technique. To improve the hydrophilicity of the polydimethylsiloxane （PDMS） stamps, the surface of the stamps was treated with 02 plasma. After that, the stamps were coated with an aqueous Col-I protein solution, dried in a soft nitrogen air stream and placed in a wet box to return damp for 1 min in order to gain high quality protein pattern. Then the stamps were kept contact with glass substrate for 10 s under the pressure of 50 g/ cm^2, which has resulted in better defined Col-I mieropatterns on the surface. The result shows that the hydrophilicity of the PDMS stamp surface is improved by 02 plasma. After damping the stamp in a wet box, the micropatterning quality of Col-I can be significantly increased.