乙肝前S1抗原、E抗原及HBV-DNA检测结果分析

Analysis on the results of pre S1 antigen,HBV DNA detection

目的分析探讨乙型肝炎病毒(HBV)3种检测方法其结果的临床意义。方法前S1抗原(pre-s1Ag)、HBV-DNA血清学检测采用酶联免疫吸附试验;HBV-DNA含量检测利用Tagman技术的荧光定量聚合酶链反应(FQ-PCR)以HBV-DNA拷贝数大于103copy/mL为阳性。结果(1)在280例HBV-DNA阳性人群中,pre-S1Ag总阳性率为94.6%,HBeAg总阳性率为63.2%,二者差异有统计学意义(P<0.05);(2)HBV-DNA拷贝数103～107copy/mL之间,pre-S1Ag的阳性率比HBeAg高,差异有统计学意义(P<0.05);(3)在HBV-DNA拷贝数超过108时,pre-S1Ag的阳性率与HBeAg相比,差异无统计学意义(P>0.05)。结论pre-S1Ag与HBV-DNA具有高度的相关性,可作为反映HBV复制及传染性的又一可靠血清学指标,pre-S1Ag与HBeAg联合检测对判断HBV复制具有更重要的临床意义。

Objective To explore the the significance of the methods to detect hepatitis B virus and its results in clinic. Methods pre-S1Ag antigen （pre-S1Ag）, HBV-DNA detection in serum was conducted by enzyme linked immunosorbent assay; and the content of HBV-DNA was determined by means of Tagman technology of fluorescence quantitative polymerase chain reaction. （it＇s positive if the HBV-DNA copy number〉1000/mL）. Results （1）Among 280 cases of HBV-DNA positive populations, pre-S1Ag positive rate was 94. 6% while the HBeAg positive rate was 63.20%, and the result is of statistical significance （P〈0.05）. （2）When the HDPFDNA copy number is be- tween 1 000-10 000 000 mL,the pre-S1Ag positive rate is higher than that of HBeAg positive （P〈0.05）. （3）When the HBV-DNA copy number is more than 100 000 000, there is no significant difference between pre-S1Ag positive rate and HBeAg（P〉0.05）. Conclusion There is a high correlation between pre-S1Ag and HBeAg. This method could be regarded as another reliably serological indicator in reflecting the HBV replication and infections. The combined detection of pre-S1Ag and HBeAg is of an important significance in determining the HBV replication clinically.