ALT测定中两种波长设置对不同浓度溶血影响程度的控制

Control of the influence of detecting wavelengths on the different degrees of hemolysis in ALT determination

目的观测不同波长设置时,3种溶血程度对丙氨酸氨基转移酶(ALT)测定的影响。方法用Sys-mexXT-1800i全自动血细胞分析仪测定血清中血红蛋白(Hb)浓度。应用OlympusAU2700全自动生化分析仪在波长设置于340nm和340/660nm时检测20例住院患者,在不溶血和溶血程度在Hb为2.0、5.0、8.0g/L时ALT指标的变化。结果应用配对设计差值均数t检验得出,波长340nm时,与无溶血比较,Hb=2.0g/L时差异无统计学意义(P>0.05);Hb=5.0g/L时显著增高,差异有统计学意义(P<0.05);Hb=8.0g/L时显著增高,差异有统计学意义(P<0.01)。波长340/660nm时,与无溶血比较,Hb=2.0g/L时差异无统计学意义(P>0.05);Hb=5.0g/L时差异无统计学意义(P>0.05);Hb=8.0g/L时显著增高,差异有统计学意义(P<0.01)。无溶血标本在两种波长设置检测结果差异无统计学意义(P>0.05)。结论一定的溶血会影响ALT检测,不同波长设置抗干扰能力不同,340/660nm优选。

Objective To observe the effects of three degrees of hemolysis in ALT determination with two different wavelengths. Methods To determine the concentration of hemoglobin in serum with Sysmex XT-1800i automatic blood analyzer, and determine the activities of ALT in serums, in which the concentration of hemoglobin has been determined（2.0 g/L,5.0 g/L,8.0 g/L） with Olympus AU2700（340 nm,340/660 nm）. The serum were collected from 20 patients in the hospital. Results By means of partnership difference t-test ,when single wavelength is at 340 nm, there is no remarkable difference（P〉0.05） between serum without hemolysis and 2.0 g/L concentration in Hb ;but there is remarkable difference（P〈0.05） between serum without hemolysis and 5.0 g/L concentration in Hb ; There is remarkable difference（P〈0.01） between serum without hemolysis and 8.0 g/L concentration in Hb. By using double wavelengths（340/660 nm）, there is no remarkable difference（P〉0.05） between serum without hemolysis and 2.0 g/L concentration in Hb ; There is no remarkable difference（P〉0.05） between serum without hemolysis and 5.0 g/L concentration in Hb ; There is remarkable difference（P〈0.01） between serum without hemolysis and 8.0 g/L concentration in Hb. There is no remarkable difference（P〉0.05） between single wavelength （340 nm） and double wavelengths（340/660 nm）if the serum is normal without hemolysis. Conclusion Control of influence on different degrees of hemolysis in ALT determination between single wavelength （340 nm） and double wavelengths （340/660 nm） is different. With single wavelength（340 nm）, the exact results of ALT activity can be made if Hb interference is low. Proper wavelengths （340/660 nm） should be selected according to the increasing Hb interferences.