鹿瓜多肽对膝骨关节炎保护作用的动物实验研究

The animal experimental study of Cervus and Cucumis Polypeptide for knee osteoarthritis

目的通过观察鹿瓜多肽对动物膝骨关节炎（OA）软骨的影响，探讨鹿瓜多肽对骨关节炎的治疗作用。方法6个月龄新西兰大白兔18只，采用随机数字表法分为3组，每组各6只，正常空白对照组：非手术，正常喂养；模型对照组：行双膝关节部分软骨缺损手术；观察组：行双膝关节部分软骨缺损手术，术后第2周开始，正常空白对照组、模型对照组不予任何处理；观察组按0．6ml／kg腹腔注射鹿瓜多肽，1次／d，术后12周处死动物。切取胫骨平台关节软骨大体观察，苏木素-伊红及甲苯胺蓝染色，检测血清、关节液。结果正常空白对照组动物模型关节软骨正常；模型对照组动物模型关节软骨缺损部分修复；观察组动物模型关节软骨缺损完全修复。观察组关节软骨形态学上明显改善，血清及关节液中超氧化物歧化酶（superox—idedismutase，SOD）分别为（117．31±11．49）mol／L、（76．28±19．11）mol／L，明显高于模型对照组[（102．37±11．54）mol／L，（57．58±17．31）mol／L]（P〈0．01）。结论鹿瓜多肽能够降低兔膝关节骨关节炎软骨的退变，通过抑制关节软骨炎症，加强关节软骨下骨的吸收应力的能力来保护关节软骨。

Objective To evaluate the effects of intraarticular and intraperitoneal injection of CCP on experimental osteoarthritis in rabits and to observe the OA pathogenesis related to free radical. Methods Animal models of osteoarthritis in knee of rabits was established. 18 rabits were divided into 3 groups： unoperated animals （group A）, opeatated animals （group B）, OA rabits that received injection of CCP （group C）. The animals had both knee joints operation and were killed 12 weeks later. Macroscopic and histologic analysis were performed on the articular cartilage. Meanwhile the activity of SOD in serum and synovial fluid were detected. Results Injection of CCP alleviated the wear and degeneration of cartilage including the formation of the osteophyte. It also improved the morphologic appearance,decreased the Markin＇s marks and remarkably increased the activity of SOD in serum and synovial fluid. Conclusions CCP can regulate the cartilage degeneration of osteoarthritis in knees,reduce the cartilage inflammation and increase the subchondral bone stiffness, showing a protective effect on articular cartilage.