原生质体融合选育乳清发酵生产燃料乙醇的酵母菌种

A Recombinant Saccharomyces cerevisiae Strain for Efficient Conversion of Lactose in Cheese Whey into Ethanol

以酿酒酵母AY-5和克鲁维酵母TY-13为出发菌株进行原生质体融合选育乳清发酵优良菌种。通过对原生质体融合条件的优化获得152株初筛菌株,经麦芽糖和乳糖杜氏管试验复筛得4株融合子。对4株融合子进行乳清发酵实验,从中选出1株发酵优良的菌株R-1,与亲本比较,发酵速度明显提高。

Intergeneric protoplast fusion between Saccharomyces cerevisiae AY-5 and Kluyveromyces marxianus TY-13 was carried out to obtain quality strains for lactose fermentation in whey. 152 fusants were selected from the fusion plates. Among these fusants, 4 strains were finally obtained through maltose and lactose Durham test. Then the 4 fusants were used for whey fermentation experiments and strain R-1 with satisfactory fermentation performance was selected finally. Compared with parent strains, strain R-1 had quicker fermentation speed.