摘要
目的:建立HPLC法测定盐酸地芬尼多片的含量。方法:色谱柱为Diamonsil C18(4.6 mm×150 mm,5μm);甲醇-水-三乙胺(55∶45∶0.2)用磷酸调节至pH4.0为流动相,检测波长220 nm。结果:在12.4~37.2μg/ml范围内,溶液浓度与峰面积呈良好的线性关系(r=1.0000);平均回收率为99.3%,RSD为1.1%(n=6)。结论:本方法简便、准确、可靠。
Objective: To establish an HPLC method for the determination of difenidol hydrochloride tablets.Methods: The chromatographic system consisted of a C18 column as stationary phase and a solution of methanol-water-triethylamine(55∶45∶0.2),pH of which was adjusted to 4.0 with phosphoric acid,as mobile phase.The detective wavelength was at 220 nm.Results: The linear range of difenidol hydrochloricde was 12.4~37.2 μg/ml(r=1.000 0).The average recovery was 99.3%,and RSD 1.1%(n=6).Conclusion: This method is simple,accurate, and reliable.
出处
《天津药学》
2009年第5期5-7,共3页
Tianjin Pharmacy