肝细胞癌P-gp定量分析与生物学特性的关系

Relationship between expression of P—glycoprotein and biological character in hepatocellular carcinoma

研究肝细胞癌 MDR1基因产物 P-gp 的表达及临床意义。方法:应用免疫组化结合图像分析定量测定47例肝癌组织和33例癌旁组织 P-gp 含量,并与肝癌生物学特性比较。结果:肝癌组织和癌旁组织均有 P-gp 表达,且主要位于肝细胞膜上。肝癌组织和癌旁组织 P-gp 含量均高于正常肝组织(P<0.01)。高分化肝癌和肝内复发肝癌 P-gp 含量分别高于低分化肝癌和未复发肝癌(P<0.01),侵袭性肝癌 P-gp 含量也高于非侵袭性肝癌(P<0.05)。结论:P-gp 表达可能成为肝癌预后判断的生物学指标。

To study the clinical significance of the expression of P-glycoprotein(P-gp)in hepatocellular carcinoma(HCC).Methods:Quantitative analysis of P-gp was made by using immunohistochemical method and microimage analysis technique in 47 tumor tissues and 33 tumor-adjacent tissues of the patient with HCC.The biological character was also analyzed.Results:Anti-P-gp immunostaining was observed both in 72 % of cases with tumor tissue and in 66% of cases with adjacent to tumor tissue.When P-gp was detected in HCC,it was mainly localized on the cellular membrane.A significant increase in P-gp level was observed in tumor tissue (P<0.01)and tumor-adjacent tissue(P<0.01)as compared with normal liver tissue.P-gp expression level was significantly higher in HCC of the well differentiated type than in that of poorly differentiated type(P<0.01). HCC with intrahepatic recurrence had a significantly higher P-gp expression level than that without intrahepatic recurrence(P<0.01).P- gp overexpression level correlated well with the invasiveness of HCC(P<0. 05).Conclusion:P-gp expression is Probably a biological marker of Prognostic value to HCC.